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1. Table below shows the volume of agarose solution required to
make the desired agarose gel (5 mm thick) for each unit tray
size. For a standard 0.7 % agarose gel, add 0.7 g of agarose to
100 ml of 1x TAE or TBE solution. The same 1x solution should
be used in the tank buffer solution.
Model
E3100
E3200
E3300
E3400
E3500
E3600
2. Add the agarose powder to a conical flask.
3. Add the appropriate amount of 1 x TAE or TBE solution from
the table above. To prevent evaporation during the dissolving
steps below, the conical flask should be covered with para-
film.
4. Dissolve the agarose powder by heating the agarose either on
a magnetic hot plate with stirring bar or in a microwave oven.
If using the microwave method, the microwave should be set
at around a 400 W or medium setting and the flask swirled
every minute. The solution should be heated until all crystals
are dissolved. This is best viewed against a light background.
Crystals appear as translucent crystals. These will interfere
with sample migration if not completely dissolved.
5. The gel must be cooled to between 50°C and 60°C degrees
before pouring.
E3xxx
E3xxx
Gel size (cm)
10x8
7x7
7x10
10x7
10x10
15x7
15x10
15x15
20x10
20x20
26x16
26x24
26x32
Volume (ml)
40
25
35
35
50
53
75
113
100
200
208
312
416
Gel preparation
Gel preparation
5 5