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• Olympus IF-550 (interference) or 45G533 (absorptive) filter

• One or more Olympus LB phase contrast objectives

Match Selector Dial with the Low-Power Objective

Select the lowest-powered phase contrast objective

available on your microscope. Rotate the selector dial

on the front of the phase contrast condenser until the

number visible on the front of the dial matches the

selected phase contrast objective (see

sure the selected number on the dial clicks in place.

Figure 1 – Set condenser dial to match the objective

Replace Eyepiece with Phase-Centering Telescope

Carefully remove the eyepiece from the right-hand

ocular tube and insert an Olympus phase-centering

telescope

in its place (see

Figure 2 – Replace right eyepiece with phase telescope

Focus the Phase-Centering Telescope

Look into the phase-centering telescope.

lighting intensity set to a comfortable viewing level, you

Use the "CT-5" for 23mm tubes and use the "CT-30" for 30mm

tubes. Note that other manufacturer's centering telescopes may be

substituted for the Olympus CT, so long as the barrel diameter of the

phase telescope is compatible with your ocular tubes.

Phase Contrast on the Olympus BH-2 Microscopes

Figure

1). Make

Figure

2).

With the

should see a bright ring of light and also a darker ring,

similar to that shown in

may be so blurry that these features cannot be readily

discerned at this point. Also note that the relative

positioning of the two rings may vary from that shown

Figure

Figure 3 – Phase telescope improperly focused

Rotate the knurled focusing ring on the phase-centering

telescope (see

Figure

4) until the image seen through

the phase-centering telescope is sharply focused (see

Figure

5).

Figure 4 – Adjust knurled focus ring to focus the image

Figure 5 – The phase telescope is properly focused

Revision 1

Figure

3. Note that the image

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