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Fig. 2
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Centering the Aperture Iris Diaphragm
1. Engage the shutter in the light path by pressing the RSHT button on the
hand switch.
2. Engage the B or IB mirror unit in the light path by pressing the MU+ or
MU– button on the hand switch.
(If these mirror units are not available, engage another fluorescence mir-
ror unit in the light path.)
3. Engage the 10X objective in the light path by pressing the objective
button, then place the U-CST centering target on the stage.
4. Release the shutter by pressing the RSHT button.
5. Move the white surface with crosslines of the U-CST until the crosslines
are overlaid on the center of field.
6. Engage the empty place (the objective cap should be removed) in the
light path.
7. Pull out the aperture iris diaphragm knob @ to minimize the aperture iris
diameter.
8. Pull out the field iris diaphragm knob ² to minimize the field iris dia-
phragm. Now the aperture iris image should be visible on the U-CST.
9. Fit the Allen screwdrivers in the two aperture iris centering screws ³ and
adjust so that the aperture iris image coincides with the crosslines.
Effects of Aperture Iris Diaphragm
The aperture iris diaphragm helps adjust the brightness of the observed
image and improve the contrast.
To perform normal fluorescence observation, enlarge the aperture iris by
pushing in the aperture iris diaphragm knob @.
}If specimen colors tend to fade due to too high excitation light, first use
ND filters to reduce the brightness, and decrease the aperture iris dia-
phragm if the ND filters are not enough.
Do not decrease the aperture iris diaphragm too much. Do not use it as
a substitute to the shutter.
(Fig. 2)