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LM
MW Microscop
py Laboratory
F. Ac
cquisition of Z
Z‐stack (z‐ser
1. A
z‐stack image
e contains mu
st
tack
but
tton to display
move the stage
m
e using the Pr
Pr
rocess Manag
ger window.
cl
ose to the co
ver slip and t
br
ring up the st
age to focus
d
bu
utton
, whic
ch will the Sta
po
osition.)
cover slip
Slide
2. Th
he program w
will automatic
ob
bjective. Clic
k Apply butto
It
will calculate
e the number
3. Cl
lick the Start
button to cap
G. Di
isplaying and
d Converting z
1. In
n the Processi
ing tab, the z‐
2. W
With the navig
gator bar
fr
ames of a z‐s
tack.
3. Yo
ou can select
a single fram
m
mouse button
in a single fra
Fr
rame from th
e context me
4. Yo
ou can also se
elect a short r
g
bu
uttons
; det
termine a new
St
tarting Positio
on and choos
Th
hen choose T
Tile View. Ma
cl
ick the right‐m
mouse button
co
ontaining only
y selected fra
y
ies)
ultiple frames
s acquired at
y Z stack pan
el. To set the
rior stage con
ntroller or by
First, lower t
the stage to fo
c
hen click Set
button
onto a deepe
er region close
art position. (
(Image acquis
Objective
e
cally recomme
end a step siz
on to accept t
this step size
f
of z‐slices
accordingly.
pture a z‐serie
es. You can co
z‐stacks
‐stack image
can be manip
me from a z‐st
ack by clickin
ame view and
d selecting Ex
enu.
range of fram
mes within the
w starting pos
sition within t
se a new end
position and
ake sure all th
e channels ar
n and select E
Extract from t
ame will be ge
enerated as a
O l y
different foc
us positions.
e Start and En
nd limits of a
clicking the a
arrowheads
ocus onto a r
region of inte
, wh
hich will be th
he End positio
e to the slide
and click Set
sition will beg
gin at the Sta
End
Start
on stage
e
ze
for z‐ser
ries according
or change as
you want an
ombine this f
function with
pulated in var
rious ways to
ng the right‐
xtract Current
t
e stack by usin
ng Set Select
the stack and
d click Set Sele
click Set Sele
ection End Po
re visible (sel
ected). Over
the context m
menu. A new
.vsi file. Save
e the image.
m p u s B X
X 5 3 U s e r
Click z‐
z‐stack,
b
b
in the
rest
on. Next,
t
rt
e
e
e
f
g to the
d apply.
multicolor im
maging. Save
generate dif
fferent views.
, you ca
an navigate d
g
ion
ection
osition.
an image,
w z‐stack
G u i d e
| 8
8
d
b
c
e the images.
.
ifferent focal