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VI. OPTICAL DATA
Objective
Type
Achromat
Magnification
4X
lOX
S40X
Sl00X'
NA
0.10
0.25
0.65
1.30
W.D.(mm)
19.87
5.40
0.39
0.11
Focal length (mml
29.20
15.98
4.31
1.81
Resolving power**{J-L)
3.4
1.3
0.52
0.26
Remarks
Spring-
Spring-
Eyepiece
loaded
loaded
WFlOX
Total magnification
40X
100X
400X
1000X
(Field
number
Focal depth
(Il)
172.5
27.60
3.03
0.66
18)
Field of view (mm)
4.5
1.8
0.45
0.18
.. Immersion objective.
*·The resolving power is obtained
if
the objective is used with the fully opened aperture
diaphragm.
•
Working distance:
The distance from the specimen or cover glass to the nearest point
of the objective.
•
Numerical aperture:
The N.A. represents a performance number which could be
compared to the
relativ(~
aperture of a camera lens. The quantity
of light which the objective receives from the object- increases with
the square of the performance number.
•
Resolving power:
The resolving power of a lens is measured by its ability to separate
two points.
•
Focal depth:
The distance between the upper and lower limits of sharpness in the
image formed by an optical system. As you stop down the aperture
iris diaphragm, the focal depth becomes deeper. The larger the N.A.
of the objective the shallower the focal depth.
•
Field number:
A number that represents the diameter in mm of the image of the
field diaphragm that is formed by the lens in front of it.
•
Field-of-viewdiameter: The actual size of the field of view in mm.
VII. TROUBLESHOOTING
Troubles
Causes
Remedies
1. Optical System
a) Field of view is cut off, or
Nosepiece did not change properly.
Slightly rotate the nosepiece until
illuminated irregularly.
it clicks into position.
Condenser is not correctly mounted
Re-insert the condenser all the way.
on the ring mount.
10