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Using the 100X Immersion Objective

Fig. 16

}The designated immersion oil should be attached to the top lens of the

100X immersion objective. Otherwise, the observed image will be unable

to be focused on.

# Always use immersion oil supplied by Olympus.

1. Focus on the specimen using all objectives, starting from the lowest-

power objective to higher-power objective.

2. Before engaging the immersion objective in the light path, place a drop

of provided immersion oil onto the specimen at the area to be observed.

3. Rotate the revolving nosepiece to engage the immersion objective and

rotate the fine adjustment knob to bring the specimen into focus.

# Since air bubbles in the oil will affect the image quality, make sure

that the oil is free of bubbles.

To remove bubbles, rotate the revolving nosepiece slightly to move

the oil-immersed objective by one reciprocation or two.

}The condenser of this microscope manifests the full performance when

oil is placed between the slide glass and the front lens of condenser. If oil

is not attached there, the observation image may become slightly dark.

4. After use, remove oil from the objective front lens by wiping with gauze

slightly moistened with absolute alcohol.

Caution in use of immersion oil

If immersion oil enters your eyes or contacts your skin, immediately

take the following treatment.

Eyes: Rinse with fresh water (for 15 minutes or more).

Skin : Rise with water and soap.

If the appearance of the eyes or skin is altered or pain persists,

immediately see your doctor.

(Fig. 16)