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顕微鏡 Olympus IX70のPDF 操作マニュアルをオンラインで閲覧またはダウンロードできます。Olympus IX70 32 ページ。 Inverted fluorescent microscope
Olympus IX70 にも：操作説明 (5 ページ)

find bright/dark areas, etc. This works on all images, not just binary.

UseRegionForBackground subtracts off the min/avg/max from a ROI defined as background.

SubpixelShift allows image or ROI movements/alignments in steps of 5%.

SegmentImage is an automated method of separating objects in images (for analysis). You can have it find stuff based

on intensity and of texture. It can be hard to get correct output.

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Process submenus

Tools to analyze and massage data.

The top section lists common image filters. Many

operations use "kernels" that pass over data and do

operations. You can create/use custom filters (Kernel tools).

FFT performs operations in frequency space such as periodic

noise/line removal (very math intensive). Remove haze

requires z stacks and subtracts off a portion of info averaged

from planes above/below the one you are working on. 2D

deconvolution is more optically precise, requiring

information about the optics used (should be already known

for data collected here) to de-haze based on optical theory.

No-neighbor is pure theory, nearest-neighbor uses data from

above/below in addition, to clear blur.

Binary images are only black+white. Mainly used for

special analysis operations.

Arithmetic calculates many operations between

images/stacks or constants (e.g. subtracting a background

image)

StackArithmetic does same but WITHIN a stack

RatioImages creates MetaFluor-like output

FlattenBackground corrects for uneven lighting when no

real background image exists

CorrectShading flattens illumination defects based on a

bright reference image.

ProduceBackgroundCorrectionImage attempts to create a

background from your image.

MorphologyFilters are used to smooth specific interests,

Output results to text files and/or Excel

Most measurements go into a data log

Object logs are for Morphometric Routine output

Summary Logs (morphometry) yet another output option (no

detail, just the means, StdDevs, etc)

LogPixelsInRegion lists intensity data for every pixel within a

ROI

EdgeLists (generally not used)