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4.2 Sample Preparation

Prepare a cell suspension according to standard procedures. Mix gently but thoroughly to ensure that the

suspension is homogenous.

Mix 10 µL of the cell suspension with 10 µL of trypan blue stain. Pipette gently.

Open a new Counting Slide. Hold the Counting Slide by its edges and load 10-12 µL of the cell sample into a

sample chamber. For easy and accurate loading, hold the pipette at a 45-60° angle to the slide. Be careful not

to over-load or under-load the sample chamber.

Insert the Counting Slide face up and sample-side first into the counting slide port of R1. The R1 can only

analyze the inserted chamber.

Do not insert the Counting Slide facedown.

A live image of the cells will appear on the screen. If an image does not appear, the cell counting slide may be

inserted incorrectly.