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Trouble

The contrast is low.

Chapter 8

Troubleshooting

Image Output to a PC Monitor

• Check that the tone is adjusted correctly with the application software.

⇒ Refer to the instruction manual for the application software.

• Check that the black level is set appropriately with the application

software.

⇒ Refer to the instruction manual for the application software.

• Check that the size of the field diaphragm is adjusted so that it

circumscribes the view field.

• Check that the size of the aperture diaphragm is adjusted to

approximately 70% to 80% of the numerical aperture of the objective

lens.

• Check that an appropriate filter is used.

When using a phase contrast microscope or an interference microscope

(two luminous fluxes or multiple luminous fluxes), insert the specified

filter. With a black and white image, contrast is improved by inserting a

green interference filter (GIF). Contrast in color images is enhanced by

inserting a filter having a complementary color to that of the specimen.

• Check that the ambient light around the microscope does not affect.

If the ambient light affects, darken the room or attach a cap to the

binocular eyepiece.

• Check that the contrast of the specimen is sufficient.

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Check point