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(6) Adjusting the exposure time

Adjust the illumination for the microscope to a level that yields a suitable exposure time (generally

1/15 to 1/250 s) using a ND filter. Or, adjust the exposure condition of the camera.

Terms

What is a ND filter?

An ND filter is a filter that affects only the amount of light passed, not the color balance of the

light. For example, an "ND2" filter cuts transmitted light in half, while an "ND16" filter reduces

transmitted light to 1/16th of actual levels.

(7) Effects of ambient light

When capturing a dark specimen with a fluorescent microscope, ambient light may enter the optical

path. Accordingly, darken the interior lighting for photomicrography. Attach the caps over the

binocular eyepiece to prevent light from entering through the eyepiece.

(8) Effects of vibration

Because photomicrography involves extremely high image resolution, a slight vibration can affect

image quality. To insulate the microscope from vibration, set it on a solid, stable surface on a solid

floor. Using an anti-vibration table compatible with the microscope will further reduce the effects of

vibration. During microphotography, be careful to avoid touching the microscope or the desk or

stand on which it is positioned.

Chapter 5

Microscope Setting

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