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3. Microscopic Procedure

Switch to any desired objective

and view the specimen.

• Re-adjust the focus.

• Use ND filters to adjust the

brightness.

• Close the field diaphragm so

that it is just outside the field

of view.

• Close the condenser aperture

diaphragm to about 70 to

80% of the objective

numerical aperture, (p. 19)

■ If a portion of the field of view is dark, try adjusting the condenser centering screws. If that does

not resolve the problem, check the following items:

• Filter insertion/removal (p. 21)

• Positioning of the revolving nosepiece (p. 35)

• Condenser focusing (p. 18)

• Full opening of the field and aperturediaphragms (p. 19, 20)

• Optical path selection for the eyepiece tube (p. 16)

• Lamp installation (p. 28)

• Revolving nosepiece installation (p. 30)

• Condenser installation (p. 30)

■ If it is not possible to focus on the specimen, check the following items.

• Mounting of the specimen (p. 35, 36)

• Release of the coarse focus stopper (p. 15)

• Thickness of the cover glass (0.17 mm is standard) (p. 35)