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Streaming imaging

Open the Stream Acquisition window with the button in

the taskbar

•

Start recording with Acquire button

•

Check if chosen configuration is ok

Tab Acquire:

• Acquisition modes:

o RAM save later to hard disk

o HD

choose name and location to save

Save as on file or separate TIF files

• Enter the desired number of frames

• Select "Initial illumination": Current Shutter

Tab Camera Parameters:

• Recording options:

o Average frames

o Number of frames to skip, during acquisition

o Don't show recording (faster when exposure time <

minimal interval time)

• Camera readout default options should be:

o Camera state: HALT

o Shutter mode: OPEN PRE SEQUENCE

o Clear mode: CLEAR PRE SEQUENCE

Multi-Dimensional Acquisition (MDA)

Combine multiple tasks: z-stack, time-lapse,

streaming, multiple positions, multiple

wavelengths, use of journals.

• Open the MDA window using Taskbar

• Select acquisition mode(s) in Main

• Continue with the tabs shown left:

• Saving: select filename and folder

• Select illumination settings

• ... options for acquisition modes

• When setup is done press acquire

• Save or load settings of experiment setup,

with Save/Load State buttons

• Live mode can be started from MDA window

Martijn de Gruiter

Manual: TIRF Microscope

Erasmus OIC

Ee-1454