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Checklist
Make sure that all the modules Pressure
•
sensor, Pump, UV and Conductivity are
calibrated.
•
Set the conductivity reference temperature to
20°C.
•
No column should be present in the flow path.
•
Immerse Buffer port A inlet in Buffer A (DM
water).
•
Immerse Buffer port B inlet in Buffer B (Acetone/
NaCl).
•
Immerse Sample valve inlet in sample (Acetone/
NaCl).
•
Fit a 1 ml Sample loop and fill with sample
(Acetone/NaCl).
•
Connect 2 m of 0.5 mm i.d. tubing to the Waste
position on the Outlet valve.
•
When performing System performance without
Fraction collector, insert the outlet valve
fractionation tubing into a pre-weighed beaker.
•
When performing System performance with
Fraction collector, connect the outlet valve
fractionation tubing to Fraction collector with at
least 5 pre-weighed tubes.
•
Make sure that the system is prefilled with DM
water.
Note: all the required observations by recording
parameters while the performance run is in
progress. Enter the observed values in the ÄKTA
start report template provided in ÄKTA start
Operating Instructions.
Operation from the Instrument
Display
Perform the following operations to run System
performance method.
Prepare system
System performance
ÄKTA start Maintenance Cue Card, 29024043 AF
ÄKTA start
Method run
method
Run
End
Exit
Acceptance criteria
Time
Activity
(min)
0
Pump wash
1
1 ml/min, 0%
B, flow through
Outlet valve,
Waste position
Repeat UV
2
Auto Zero
3
5 ml/min
4
1 ml/min,
Sample
valve, Sample
position
7
1 ml/min,
Sample valve,
Buffer position
10
Request switch
Injection valve
to Inject
position
13
Request switch
Injection valve
back to Load
position
15
Start gradient,
0-100% B in
10 min, start
fractionation/
collection
19
End
fractionation
1
20
End collection
25
End gradient,
stay at 100% B
Check
Aproved
interval
Wash valve
Mobile
position
phase out
through
Waste
Back pressure
≤ 0.05
MPa
Back pressure
0.06 - 0.2
MPa
UV level
± 10 mAU
Cond. level
± 1 mS/cm
Max UV level
300 - 380
mAU
Max Cond.
65 - 95
level
mS/cm
Max UV level
300 - 380
mAU
Max Cond.
65 - 95
level
mS/cm
Weigh fraction
0.8 - 1.2 g
no 2, 3 and 4
Max diff.
0.1 g
between
fractions
2
Weigh beaker
4.2 - 5.8 g
Gradient
Straight,
no
negative
dips.
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