DORIC BFMC Gen3 사용자 설명서 - 페이지 3

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Introduction

Doric's Bundle-imaging Fiber Photometry System Generation 3 (BFMC Gen3) is a fully integrated, oﬀering a hassle-free,

plug-and-play solution, that represents an elegant alternative for multiple site measurements. To record green and

red photometry signals, the BFMC uses built-in LEDs to illuminate the entire sample port, and a CMOS sensor to

simultaneously image the ﬂuorescent signal from every ﬁber within the bundle. The ﬂuorescent light collected from

each ﬁber within the bundle creates circular spots on a CMOS sensor. The electrical read-out from pixels within

each ﬁber image correlates with the calcium activity of the corresponding brain site within Doric Neuroscience Studio

(Fig.1.1b). The system is available for single and dual color measurements with isosbestic reference excitation (Fig.1.1a).

(a) BFMC system (Fan Out conﬁguration)

(b) Doric Neuroscience Studio BFPS interface

Figure 1.1: Bundle-Imaging Fiber Photometry System (BFPS) overview