BD FACSJAZZ 사용자 설명서 - 페이지 5
{카테고리_이름} BD FACSJAZZ에 대한 사용자 설명서을 온라인으로 검색하거나 PDF를 다운로드하세요. BD FACSJAZZ 9 페이지. Satchmo stanford shared facs facility
페이지 인쇄Accudrop Beads
1. Adjust the piezo amplitude such that there is a proper gap and break of
point. This can be monitored in the third panel. The stream should look
similar to this.
2. Click on the PLATES button to turn on the plates.
3. Click on TEST STREAM in the sort settings window. (You should see a 2
drop to the left of your stream in the 3
4. Click FLASH CHARGE and adjust the piezo amplitude till the second drop
stays out to the left.
5. Click SHORT FLASH and adjust the piezo amplitude till the deflected drop stays out to the left.
6. Vortex and load beads on to sample port and press SAMPLE and press BOOST to get them sample
running.
7. Press ACQUIRE in the acquisition bar.
8. Adjust the event rate to between 1000events/second and 1500events/second by adjusting the offset
to about 1.5 PSI
9. In the Sort Layout window, make sure the sort device
says Accudrop Setup, and it is sorting P1 to the left
10. Make sure the sort mode is 1.0 drop pure.
11. Click ACCUDROP in the Pressure Console to turn on
the accudrop filter.
12. Click START in the sort layout window.
13. Adjust the drop delay value until you only see the left
stream visible and the center stream disappears.
*Should look like the images below*
14. Click ACCUDROP in the control window to turn off the accudrop
filter.
15. Click STOP to stop the sort.
16. Press SAMPLE to stop the beads from running.
17. Remove the beads from the sample port.
18. Press BACKFLUSH, to remove the beads from the sample line.
lasted updated by MW on 11/10/2017
window of the streams.)
rd
*like the image below*
nd
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