Olympus BHA-P 사용 설명서 - 페이지 14

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Centering the Stage

I )

Looking througlk l l i c eyeplece ancl objective 10X, cleter~nine solme particular p o i n t , as

y o u like,

I n

the specimen irnage and coincidii thrs point w i t h thc center of the cross

hairs o f the eyepiece.

Rotating the stage, coincide the center of

1 1 1 1 1

the rotation o i the specimen's p o i n t g ~ i t h

I - -

>--

the center of the cross hairs by means o f

t w o centering screws

[I?

provided o n tlie

stage. (Fig. 51

Repeat this procedure u n t i l the centra-

tion is secured.

Centering the Objectives

This centration i s necessary t o all the PO objectives except

the pre-centered objective POlOX.

1 ) Connect a centering knob

t o each centering screw of

the circular rotatable stage. (Fig.

6 )

21 By means of these t w o centering screws, coincide the

centers o f the cross hairs and the rotation o f the

specimen.

3) A f t e r complete centration, remove the centering knobs.

Fig.

7. Use o f Iris Diaphragms

)

Aperture iris diaphragm

Adjust the opening of the aperture iris diaphragm

according to the various conditions scrch as the numeri-

cal aperture o f the objective, irnage contrast, depth of

focus, and flatness o f field. Generally i t is often prefera-

ble t o stop do\nln the aperture iris diaphragm to the 70%

or 80% o f the N . A . o f the objective.

A f t e r the eyepiece is removed f r o m the observation

tube, if necessary, look through the observation tube

and check the opening of the aperture diaphragm at the

objective pupil.

Field iris diaphragm

The field [!-is diaphl-agm controls the diarnetel- of tlie ray bcrndle ~ n l p i n g i n g o n the speci-

lneri s ~ ~ r f a c e and thus increases irriage definition.

Generally, i t is prefel-able t o slightly increase the diameter o f the field iris diaphragm u n t i l

i t is just outside the field of view.