Olympus Fluoview-1000 사용자 설명서 - 페이지 49

{카테고리_이름} Olympus Fluoview-1000에 대한 사용자 설명서을 온라인으로 검색하거나 PDF를 다운로드하세요. Olympus Fluoview-1000 50 페이지.

페이지 인쇄

1. Add a Scale Bar to Your File

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

Table 6. Dichroic Mirrors and Barrier Filters

Excitation

BS 20/80

DM 405/488

DM 405/488/561/633 SDM 560

DM 458/515

DM 405-440/514

DM 558/561

The excitation dichroic controls which laser lines can be employed at the same time. For instance, the

488 and 515 lines cannot be combined because the 27 nm gap between them does not provide a

sufficiently wide window for green fluorescence to return through the dichroic mirror. About 10 nm on

each side of the potential emission window is needed to allow for filter efficiency.

Table 7. Laser Power through dichroics, measured at the lens turret, 100% output

Dichroic Mirror

BS20/80

BM405/488

BM405/488/561/633 414 µW 2.6 µW

BM458/515

BM405-440/515

BM458/561

The important points for this table are:

1) Most dichroics will reflect more lines than stated

2) Non-optimal lines may leak into detectors.

The BS20/80 dichroic reflects 20% of the incident excitation light and passes 80% of the reflected light

to the detection optics.

Table 8. Index of Refraction for Common Mounting Materials

Be aware that refractive index changes with temperature and wavelength.

May 11, 2011

Olympus Fluoview-1000 User's Guide

Dichroic Mirrors

Channel 1 Channel 2 Channel 3 Channel 3

Mirror

Glass

SDM 640

SDM 510

SDM 560

SDM 490

None

405 nm 458 nm

161 µW 9.4 µW

414 µW 1 µW

306 µW 32 µW

414 µW 28.8 µW 0.6 µW

362 µW 30.4 µW 1.6 µW

Medium

Air

Water

Glycerol

Immersion Oil

Cover Glass

Mirror

BA 655-755 BA 655-755

Glass

BA 585-685 None

SDM 560

None

SDM 640

None

488 nm

515 nm

23.5 µW 50 µW

102 µW

16 µW

104 µW

2.4 µW

3 µW

161 µW

171.2 µW .54 µW

4.6 µW

Refractive Index

1.000

1.333

1.473

1.515

1.518-1.525

Barrier Filters

Channel 4

None

561 nm 633 nm

45 µW

98 µW

1.2 µW

20.4 µW

114 µW 280 µW

10 µW

7.6 µW

1.6 µW

164 µW 7 µW