Olympus FV3000 운영 매뉴얼 - 페이지 34

{카테고리_이름} Olympus FV3000에 대한 운영 매뉴얼을 온라인으로 검색하거나 PDF를 다운로드하세요. Olympus FV3000 38 페이지.
Olympus FV3000에 대해서도 마찬가지입니다: 안전 매뉴얼 (16 페이지), 운영 매뉴얼 (5 페이지)

페이지 인쇄

Processing: Unmixing（１）

②

④

⑤

Spectral Image Unmixing

〜by specifying dyes

③

④

⑥

⑧

⑥Select the mode

⑧Select the file

★You can save Dye

profile data which is

specified by ROIs.

(How to use this file?

→See page 32⑦）

⑦

① Press the

to "Viewer mode".

② Select [Analysis] in [Tool Window]

menu. [Analysis] Tool Window

appears.

③ Select [Single process] mode, press

the [Post Processing] button.

④ Press the

assigned item and select [Spectral

Deconvolution] in [Processing Item].

⑤ In [Input] in [Input / Output setting],

select the image for image processing.

⑥ In [Mode] in [processing Property],

select "Spectral Image Unmixing".

⑦ Specify multiple ROIs on the regions

where only the target fluorescence

dye locates to acquire the spectral

data for image processing.

⑧ In [DYE0 ROI File Name], select the

file name of the image on which the

ROI was specified in ⑦.

⑨ In [DYE0 ROI Index], select the first

ROI specified in ⑦.

⑩ Repeat ⑧⑨ to register all ROIs.

Press the

fluorescent separation process.

button to switch

button to reset the

⑥

⑧⑨

button to start the

⑩