Olympus U-UCD8 사용 설명서 - 페이지 35

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5-4 Centering the Condenser

Field iris diaphragm image

Eyepiece field of view

Fig. 13

Also refer to the instruction manual for the microscope in use.

Center the condenser in accordance with the following pro-

cedure.

1. Rotate the turret to select the BF brightfield observation light

path (with no optical element engaged).

2. Pull out the polarizer handling knob to disengage the polar-

izer from the light path.

3. Switch the top lens into the light path.

4. Rotate the aperture iris diaphragm lever clockwise to open

the aperture iris diaphragm.

5. Fully open the field iris diaphragm of the microscope.

6. Place the specimen on the stage, rotate the revolving nosepiece

to swing in the 10X objective, and bring the specimen into focus.

7. Reduce the microscope's field iris diaphragm opening until

the diaphragm image can be seen.

8. Looking through the eyepiece, raise the condenser almost all

the way up to bring the iris diaphragm image into focus.

9. Gradually opening the field iris diaphragm, bring the reduced

image into the center of the eyepiece field of view, by adjust-

ing the condenser centering knobs of the microscope. (The

reduced polygonal image of the diaphragm should become

inscribed in the circle which indicates the field of view.)

10. After the centering is completed, continue to open the field

diaphragm slightly until its image circumscribes the field of

view.

U-UCD8

(Fig. 13)

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