Nikon Eclipse Ti Series 사용 설명서 - 페이지 16

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Chapter

Assembly

Wnen using phase contrast microscopy

lf you also wish

perform phase contrast microscopy, install the phase

conlrast

obiectives and

the

condenser

cassettes

for

phase

contrasl

observation that correspond to the

condenser

lenses

be used, as

described

the

microscope instruction

manual.

Before beginning observation, be sure

center the

annular

diaphragm.

(Refer to the microscope instruction manual.)

The

assembly

procedure

now complete.

you

require a very precise vibraîion direction adjustment, proceed with step

lil

Optical System Direction Adiustment (Adiustment of vibration direction)

To assure

thal

DIC images can be observed at optimum contrast,

the

vibration direction of

lhe

polarizer and

analyzer must

be adjusted correctly.

Focus and center

lhe

system condenser

describ€d

the microscope instruction

manual.

Bring

the A

(empty)

cassette

into

the optical

path.

When

using

the

condenser

slider,

remove the

condenser

cassette

that is

the

optical path so

the

slider

is empty.

Bemove the polarizer,

analyzer, and

objective

DIC prism

from the

optical path.

When

using

high

condenser:

order

focus the

field

aperture

diaphragm

image on the

specimen surface,

and in order

avoid

striking lhe

spgcimen with the

condenser

lens, move

the condenser

lens

slightly

closer than

the

subiect distance

for each

condenser

lens, and

then

bring

the specimen

into focus

while

raising

the

condonser lens. When

using a

oil-immersion

type

condenser,

also

check

page

Remove

the objective

DIC prism

that

ls located

directly

below

the

4Ox

obiective.

Swilch to

the

4Ox

objective and bring

the specimen

into focus.

Move

the stage

so that the

specimen

moves

oui

of the

viewfield.

In its

place,

bring an almost

dust-free

area

into

the viewfield.

(There musî

no dust in the whole

viewfield.)

Turn

the Bertrand

lens in/out lever

to the [Bl

position

bring

the Bertrand lens

into the optical

path

that the

obiective's

€xit pupil (the bright

circle)

can be

observed. (lf your

eyepiece

tube

has no Bertrand

lens in/out lever,

install

cenlering telescope

instead of an

eyepiece and rotate

its eyepiece part

focus

the obiective's

exit pupil.)

Vibration

dirgcton lgvgr

Vlbradon

dirgclion

lgver

damp scf€r',

(bottom)

Figurc

4-9

When

using the

T-A2 analyzer, push

the

analyzer

to the

second clickstop so

that

it is in

the

optical path.

Loosen

the

clamp

screw

and align

the

index on the

vibration

direction

lever with

the

notch.

When using

the

epi-fl

filter turret

attached with the

analyzer

block, place

the analyzer block

into

the

optical path.

Teble

{-1

.gmm (including slide glass with

thid(rless of

.2mm)