Nikon A1+ 사용자 설명서 - 페이지 19

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Nikon A1+에 대해서도 마찬가지입니다: 표준 운영 절차 (15 페이지)

Tile-Scan Experiments

Basic Tiling

1. Enable the Large Image tick box in the ND

Acquisition panel

2. Choose whether the tiled area is defined by the

number of consecutive fields of view or by

absolute area (mm x mm)

3. Enable the Stitch option if you want the output image to be the montage of all the

tiles joined together

4. Select an overlap of between 10-15%

5. You can also enable any of the other ND Acquisition tabs to combine experiment

types i.e. tiling z stacks or time lapse experiments

Select Run Now to start the acquisition

Advanced Tiling

1. Select Acquire>Scan Large Image from the top menu

2. In the Capturing section select the lens to use to capture a low magnification

overview (Macro image) from which you will decide how big an area you will tile at a

high magnification (Scanning).

3. At this stage you can also choose the high magnification lens to be used for the

actual tile scan (Scanning)

4. You can see the live image and choose your position to tile by pressing the preview

button

5. Select Capture in the Stage Overview with Macro Image

section. This will capture a single image at the stage's current

location and display the macro image result.

6. Red squares will appear on the image representing the fields

of view to be scanned at the currently selected Scanning lens.