Nikon ECLIPSE 80i 사용 설명서 - 페이지 23

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Adjusting the Condenser Position

Adjust the condenser (focusing and centering) so that the light passing through the condenser

forms an image at the correct location (center of the optical path) on the specimen surface.

(1) Perform steps 1) to 10) in "Bright-Field

Microscopy" in the "Microscopy" instruction

manual to focus on the specimen using the

10x objective.

(2) Stop down the field diaphragm to the

minimum setting.

(3) Turn the condenser focus knob to form the

field diaphragm image on the specimen

surface.

(4) Turn the condenser centering screws so that

the field diaphragm image is positioned in the

center of the field of view.

(5) Set the 40x objective in the optical path. Turn

the coarse/fine focus knobs and focus on the

specimen.

(6) Turn the condenser focus knob to form the

field diaphragm image on the specimen

surface.

(7) Adjust the condenser centering screws until

the field diaphragm is at the center of the

eyepiece field of view. This is easiest if you

set the field diaphragm aperture to slightly

smaller than the eyepiece field of view.

Individual Operations

11 Adjusting the Condenser Position

Field diaphragm

Eyepiece field of view

Turn the field diaphragm knob and

stop down the field diaphragm to its

minimum setting.

Condenser focus

knob

Field diaphragm

Eyepiece field of view

Condenser centering

screws