Nikon Live Cell 2 매뉴얼 - 페이지 7

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Viewing your sample through the eyepiece:

The light-path and filter settings are stored in Elements using so-called optical

configurations. These are accessed via the OC Panel (Fig.16). The optical

configurations are grouped into categories to control viewing through the eye

port, viewing with the DS-Qi2 (sCMOS) camera (used for epi-fluorescence and

transmitted light imaging) and with the colour DS-U3 camera (e.g. for

histological stainings).

To view your sample through the eye-port, select one of the configurations

from the 'eyes' group of configurations. These configurations:

1. Set the light path to EYE

2. Set the filter in the turret to that relevant to the dye being used.

3. Make sure that the fluorescence lamp is turned on and use the

microscope base for control of the fluorescence shutter.

4. Transmitted light imaging can be achieved using the relevant OC and

using the lamp on/off but on the left hand side of the microscope;

intensity can be adjusted using the dial. For transmitted light imaging

you can select from a number of configurations: phase imaging 1 for

objectives up to 20X, phase imaging 2 for the 40X, DIC for 60X and

100X and open for standard brightfield.

Nikon Imaging Centre @ King's College London

Nikon

King's College London

Imaging Centre

Fig. 15

Fig. 16