Etaluma LS460 시작 매뉴얼 - 페이지 10

{카테고리_이름} Etaluma LS460에 대한 시작 매뉴얼을 온라인으로 검색하거나 PDF를 다운로드하세요. Etaluma LS460 14 페이지. Lumascopes with lumaview (for use with ls560, ls460, ls600, ls620, ls720)

페이지 인쇄

For phase contrast using the Phase Contrast Accessory (use appropriate Slider phase ring for objective,

Section I.E.), check Brightfield in Manual Image and turn the phase condenser iris (on front of

condenser) to the left to the fully open position. Turn Exposure and Gain to very low. Increase

Illumination (%) until a mid-gray tone is seen in the Live Image Window.

E. Focusing

1. Unless approximate sample focus level is known, it is best to initially raise objective to maximum height

(without hitting sample) and then turn focus knob counter-clockwise until sample comes into focus.

Ensure you are focusing on correct sample plane and not another surface, for example, back side of a

microscope slide. If uncertain, place a similar but easy to focus sample on stage, focus, and then switch

to new sample.

2. To facilitate the focusing process, click on the Zoom icon on the Left Tool Bar (sixth from the top). The

Live Image Window will now show the approximate middle half of the Field of View (FOV) across a

wider window. Click the Zoom icon again to return to the entire FOV.

3. If necessary, increase or decrease LED brightness and/or Gain as needed.

F. Snapping Images

1. When the desired field has been illuminated and focused in Live Image Window (immediately above),

click the Camera icon in the Left Tool Bar (third from the top) to snap the image. If the Zoom function

was used to focus, the image captured will include the entire FOV observable with the Zoom off. If

"Click" sound for manual image snap was checked in Instrument Setup, a click will sound when each

image is snapped.

2. If Manual image autosave was checked in Instrument Setup, images will be saved automatically using a

time-based numbering system (see Section II.C.7.f. about Instrument Setup for more information). If

Manual image autosave was not checked, a Save As dialog box will open after the image was snapped

(again see Section II.C.7.f. above). Images will be saved in the Etaluma folder formed automatically

whenever Lumaview is downloaded and installed. See Appendix B for the automatic subfolder

directory that comes along with the Etaluma folder.

3. To review the last image, click the Folder icon on the Left Tool Bar (bottom icon) to launch Windows

Explorer and open the most recent Destination Folder.

G. Generating Composite Images

There is no automatic compositing function in Lumaview for LS560 and LS460. To generate composite

images of green fluorescence with phase contrast or brightfield captured on an LS560 or LS460, use an

image analysis program such as Lumaquant, Fiji/Image J, or similar program.

H. Videos from Time-Lapse and Live Video

Etaluma provides several options for compiling videos from time-lapse and live video:

2017.11.20

LS560/460 Startup Guide