Clontech PT3139-1 사용자 설명서 - 페이지 3
{카테고리_이름} Clontech PT3139-1에 대한 사용자 설명서을 온라인으로 검색하거나 PDF를 다운로드하세요. Clontech PT3139-1 20 페이지. Advantage-hf pcr kit
페이지 인쇄I. Introduction
The Advantage
signed to deliver Pfu -like fidelity in the amplification of cDNA or genomic
templates.
High fidelity and efficiency
While Pfu polymerase is known for its exceptional fidelity, it suffers from subop-
timal efficiency that can be problematic in many PCR applications. In contrast, the
Advantage-HF PCR Kit offers Pfu -like fidelity combined with the efficiency
required to amplify DNA fragments of up to 2.5 kb. These benefits are the result
of reformulation of several components in CLONTECH's Advantage PCR
Enzyme Systems. The Advantage-HF Polymerase Mix combines KlenTaq (a 5'-
exonuclease-deficient variant of Taq polymerase) with a proofreading poly-
merase and TaqStart
Advantage-HF thus combines the benefits of Pfu and the Advantage Enzyme
System to deliver a high-fidelity enzyme system.
The HF Advantage
The accuracy of Advantage-HF is compared to other enzymes and enzyme mixes
in Figure 1. Using a genetic assay that measures nucleotide misincorporation,
Advantage-HF rivals Pfu in fidelity. This fidelity assay is based on amplification of
an E. coli ribosomal protein gene (Mo et al. , 1991). Mutations in this gene often
confer streptomycin resistance on the host. Upon introduction of the amplified
DNA into E. coli , the ratio of total transformants to streptomycin resistant
Figure 1. Comparison of fidelity of Advantage-HF and other PCR systems. The fidelity of
Advantage-HF compares favorably with that of the Pfu enzyme and is significantly higher than that of
other enzyme systems. Accuracy assay is based on 25 cycles of amplification (see text).
TEL:415-424-8222 or 800-662-CLON
FAX:415-424-1064 or 800-424-1350
®
-HF (High-Fidelity) PCR Kit is a KlenTaq-based system de-
TM
Antibody to provide a superior level of specificity.
500
400
300
200
100
19
0
Taq
Advantage Advantage
Technical Support
435
385
67
Pfu
HF
Enzyme
CLONTECH Laboratories, Inc.
71
Taq +
Pwo
Protocol # PT3139-1
Version # PR76834
page
3