AmScope B120 User Manual - Page 10

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120 Series

1. Adjust the microscope head so that it is in a comfortable position for observation.

2. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyeubes and ro-

tating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both eyes.

(mounted on the Siedentopf head).

3. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly

in place with the metal slide holders (clips) of the mechanical stage.

4. Using the mechanical stage controls (vertical pillar with dual knobs under the right side of the stage), center the

specimen over the stage opening, lining it up with the light and the objective lens.

5. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of

light is achieved.

1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo-

cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each

time.

2. When using the 100x objective, a drop of immersion oil should be placed between the cover slip and the objec-

tive to minimize distortion caused by air. Always be sure to wipe the oil off of the objective with either lens cleaning

paper or a nonabrasive, lint free cloth.

3. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use

the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.

Note: You may loosen the limit-stop (located rear of the stage as a vertical thumb screw) in order to give yourself

the full range of motion for fine tuning the focus.

4. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.

Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if moving the

stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when

it is off you will be able to damage the microscope. For safety, when using the 40x and 100x objectives, engage the

limit stop once you have it close or in contact with the objective (if using oil contact is required) to prevent addi-

tional movement and damage.

Operation