Carolina 3M Petrifilm ColiformCount Plates Instruction Manual - Page 5

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4. Roll the top film onto the bottom film. Do not drop the top film down

(fig. 4).

5. With the flat side down (not the side with the circular ridge), place the

spreader on the top film over the inoculum (fig. 5).

6. Gently apply pressure on the spreader to distribute the inoculum over

a circular area. Do not twist or slide the spreader (fig. 6).

Figure 4

Figure 5

Figure 6

Ridge