ATLAS BIOTECNOLOGIES AB1000 Instruction Manual - Page 4

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The pipette is a high quality instrument which offers
excellent accuracy and precision.
The accuracy and precision (repeatability) of liquid vol-
ume depend on the quality of tips used. The values for
accuracy and precision given in the table below were
obtained using A.B.T. tips.
Volume
Model
Cat. no.
[μl]
0.2
AB2
5661
1.0
Max
2.0
Min
0.5
AB10
5662
5.0
Max
10.0
Min
2
AB20
5663
10
Max
20
Min
5
AB50
5667
25
Max
50
Min
10

AB100

5664
50
Max
100
Min
20
AB200
5665
100
Max
200
Min
50
AB250
5660
125
Max
250
Min
100
AB1000
5666
500
Max 1000
Min
500
AB5000
5668
2500
Max 5000
Min
1000
AB10000
5669
5000
Max 10000
The accuracy and precision are obtained, using a gravi-
metric method, performing at least 10 measurements of
distilled water at a temperature of 20±1°C, according to
EN ISO 8655 standard.
The pipette design enables the user to perform the
recali bration process according to the rules presented in
section 8.
3
Accuracy
Precision
Tip
[%]
[%]
μl
± 12.0
± 6.0
± 2.7
± 1.3
± 1.5
± 0.7
10 μl
± 4.0
± 2.8
± 1.0
± 0.6
± 0.5
± 0.4
± 3.0
± 1.5
± 1.0
± 0.5
± 0.8
± 0.3
± 2.5
± 2.0
± 1.0
± 0.6
± 0.8
± 0.4
200 μl
± 1.6
± 0.80
± 0.8
± 0.24
± 0.8
± 0.20
± 1.2
± 0.60
± 0.8
± 0.25
± 0.6
± 0.20
± 1.0
± 0.4
± 0.8
± 0.3
300 μl
± 0.6
± 0.3
± 1.6
± 0.40
± 0.7
± 0.20
1000 μl
± 0.6
± 0.15
± 1.2
± 0.50
± 0.6
± 0.20
5000 μl
± 0.5
± 0.15
± 2.5
± 0.6
± 0.8
± 0.3
10000 μl
± 0.5
± 0.2

2 - SETTING THE VOLUME

The counter displays three figures to be read from top to
bottom. Additional to the figures on the lower wheel are
printed graduations to enable a volume setting in the
range increment of each pipette model.
Examples of the meaning of the black and red figures:
Counter
Model
readings
—
|
|
1
—
|
|
AB2
2
—
|
|
5
—
—
|
|
0
—
|
|
AB10
7
—
|
|
5
—
—
|
|
1
—
|
|
AB20
2
—
|
|
5
—
—
|
|
0
—
|
|
AB50
3
—
|
|
8
—
—
|
|
0
—
|
|
AB100
7
—
|
|
5
—
—
|
|
1
—
|
|
AB200
2
—
|
|
5
—
—
|
|
2
—
|
|
AB250
4
—
|
|
5
—
—
|
|
0
—
|
|
AB1000
7
—
|
|
5
—
—
|
|
1
—
|
|
AB5000
2
—
|
|
5
—
—
|
|
0
—
|
|
AB10000
7
—
|
|
5
—
The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the black adjustment
knob (Fig. 1B). To attain the maximum accuracy, set
volume must be approached from a higher value by
diminishing counter readings.
ENGLISH
Set
Increment
volume
1.25 μl
0.002 μl
7.5 μl
0.02 μl
12.5 μl
0.02 μl
38 μl
0.2 μl
75 μl
0.2 μl
125 μl
0.2 μl
245 μl
0.2 μl
0.75 ml
2 μl
1.25 ml
10 μl
7.5 ml
20 μl
4