Active Motif 53082 Manual - Page 10

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The crank is then turned to slowly raise the stand up the

rod. When the probe begins to enter the Alignment Tool,

you may need to adjust the position of the stand so that

the probe is centered in the tool. Simply loosen the two

thumb screws underneath the stand, use one hand to

move the stand forward/backward and left/right until

the probe is centered, then re-tighten the thumbscrews.

You may need to refine the position again slightly when

you test with a tube in the Tube Cooler (right).

Continue cranking the stand up in order to set the probe

to its chosen depth. The line that is etched into the

Alignment Tool is the approximate depth of a 1.5-2.0 ml

1 . 5 ml

tube; the bottom of the Alignment Tool is the approxi-

setting

mate depth of both a 15 ml and a 50 ml tube.

Gently crank until the tip of the probe is either even

with the etched line (if you will be sonicating in micro-

centrifuge tubes) or until it gently touches the bottom

15 / 50 ml

settings

of the Alignment Tool (if you will be sonicating in 15 ml

or 50 ml tubes, as shown to the right).

The platform is now set so that the probe will touch the bottom of the tube, which is not desired.

You must adjust the platform slightly so the probe does not touch the tube during sonication. Use

the crank to slightly lower the platform so the probe tip is the distance from the bottom of the

tube desired for your experiments. This level will depend on the volume of the samples that will

be sonicated, so you must set the final depth based on the sonication protocol that will be used.

After the probe has been positioned to the desired depth, the settings should be refined using a

tube in the Tube Cooler. Because of slight variations in the shape and thickness of tubes from

various manufacturers, the Alignment Tool may not provide you with the exact settings that will

work with your tubes.

To refine the settings, press in the latch, lower the platform, remove the Alignment Tool, place a

tube that will be used for sonication in the Tube Cooler (being certain it is all the way in the cool-

er), then place the cooler in the stand. Raise the platform back up until it is against the Stop Collar.

You may now need to use the thumbscrews again to slightly re-adjust the stand so the probe is

centered exactly in the tube. Lift the tube up and move it around slightly to verify that the probe

tip is at the desired height from the bottom of the tube. Adjust the stand height if needed.

It is important that you now write down the number on the counter . This setting will be used for

all experiments. While all you need to do to set the probe at this position is to move the platform

up and down, if another user raises or lowers the platform using the crank, or if they change

the counter, your positioning will be lost. By recording the counter value you can replicate your

desired settings. Simply re-zero the platform as before, then crank the platform up to your setting.