3B SCIENTIFIC PHYSICS 1012404 Instruction Manual - Page 2

Browse online or download pdf Instruction Manual for Microscope 3B SCIENTIFIC PHYSICS 1012404. 3B SCIENTIFIC PHYSICS 1012404 4 pages. Binocular polarisation microscope

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Condenser: Abbe condenser N.A.1.25 NA 0.65

with iris diaphragm , filter holder and blue filter,

focussed via rack and pinion drive

Dimensions: 240 x 190 x 425 mm³ approx.

Weight: 6 kg approx.

3. Unpacking and assembly

The microscope is packed in a molded styro-

foam container.

•

Take the container out of the carton remove

the tape and carefully lift the top half off the

container. Be careful not to let the optical

items (objectives and eyepieces) drop down.

•

To avoid condensation on the optical com-

ponents, leave the microscope in the original

packing to allow it to adjust to room tem-

perature.

•

Using both hands (one around the pillar and

one around the base), lift the microscope

from the container and put it on a stable desk.

•

The objectives will be found within individual

protective vials. Install the objectives into the

microscope nosepiece from the lowest

magnification to the highest, in a clockwise

direction from the rear.

•

Put the head onto the top of the stand and

tighten the head-lock-screw. Insert the eye-

pieces into the tube.

•

Insert the analyser into the slot on the re-

volving nosepiece.

•

Insert the condensor with polariser and tigh-

ten the lock-screw.

4. Operation

4.1 General information

•

Set the microscope on a level table.

•

Place the object to be observed in the centre

of the specimen stage. Use the clips to fas-

ten it into place.

•

Connect the mains cable to the net and turn

on the switch to get the object illuminated.

•

Make certain that the specimen is centered

over the opening in the stage.

•

Adjust the interpupillary distance so that one

circle of light can be seen.

•

Make the necessary eyepiece dioptre ad-

justments to suit your eyes.

•

To obtain a high contrast, adjust the back-

ground illumination by means of the iris dia-

phragm and the variable illumination control.

•

Rotate the nosepiece until the objective with

the lowest magnification is pointed at the

specimen. There is a definite "click" when

each objective is lined up properly.

NOTE: It is best to begin with the lowest power

objective. This is important to reveal general

structural details with the largest field of view

first. Than you may increase the magnification

as needed to reveal small details.

To determine the magnification at which you are

viewing a specimen, multiply the power of the

eyepiece by the power of the objective.

•

Adjust the holding brake to give a suitable

degree of tightness in the focusing mecha-

nism.

•

Adjust

the

coarse-focusing-knob

moves the stage up until the specimen is fo-

cused. Be careful that the objective does not

make contact with the slide at any time. This

may cause damage to the objective and/or

crack your slide.

•

Adjust the fine-focusing-knob to get the im-

age more sharp and more clear.

•

Colour filters may be inserted into the filter

holder for definition of specimen parts.

Swing the filter holder out and insert colour

filters.

•

Always turn off the light immediately after

use.

•

Be careful not to spill any liquids on the mi-

croscope.

•

Do not mishandle or impose unnecessary

force on the microscope.

•

Do not wipe the optics with your hands.

•

Do not attempt to service the microscope

yourself.

4.2 Using the polarisation equipment

•

Insert the analyser into the slot on the re-

volving nosepiece.

•

Rotate the polariser until the planes of the

polariser and the analyser are exactly crossed,

so that one sees a black background.

Any object with a doubly-refracting (birefringent)

structure should now appear brightly illuminated

against the dark background. If that does not

occur, it is possible that the direction of light

vibration of the object coincides with the polari-

sation direction. Whether or not that is the case

can be tested by rotating the polariser or the

specimen itself.

A birefringent object, when rotated continuously,

shows up brightly after each 90° rotation and is

dark between these positions. In contrast, ob-

jects that are isotropic and not birefringent re-

main dark in all positions.

which