Olympus BHS-313 Instruction Manual - Page 14

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Condenser Adjustment

1, Condenser Centration

1) Stop down

the field iris diaphragm with

knurled

ring ®

by rotating in the direc-

tion of the arrow. (Fig. 14)

2) Use

the

condenser

height

adjustment

knob @

to move the condenser up and

down

until

image

of the field dia-

phragm

can

seen

clearly

in the eye-

pieces.

The

rotation of the knob

in the

direction

of the arrow lowers the con-

denser.

Field iris diaphragm image

Field of view

Fig. 15

Field Iris Diaphragm

The field iris diaphragm controls the diameter of the ray bundle impinging on the speci-

men

surface

and

therefore,

stopping

down

the field diaphragm

until

it is slightly

larger than the field of view, it can reduce stray light, which

in turn increases image defi-

nition and contrast.

Aperture

Iris Diaphragm

In order to achieve optimum

objective performance, the opening of the aperture iris

diaphragm

should be matched to the numerical aperture of the objective in use.

It is

often preferable,

however,

to stop down

the aperture diaphragm

slightly more than in-

dicated by the objective N.A.

This will result in better image contrast, increased depth of

focus and a flatter field.

@ After completing

focus adjustment, re-

— 80%

move one of the eyepieces from the obser-

;

7207508

vation

tube

and

look

into the empty

eyepiece tube. As you stop down the aper- DPening of the

ture iris diaphragm, the image of the iriş aperture diaphragm 人

diaphragm

can

seen

in the objective

pupil,

Adjust

the

opening

of the

dia-

phragm to match the N.A. of the objective

in use. If the specimen is low in contrast,

it is recommended

to stop down to 70%

~80% of the objective N.A. (Fig. 16)

Objective exit pupil

Fig. 16