Olympus BHS-313 Instruction Manual - Page 14

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Olympus BHS-313 Instruction Manual
D.
Condenser Adjustment
1, Condenser Centration
1) Stop down
the field iris diaphragm with
knurled
ring ®
by rotating in the direc-
tion of the arrow. (Fig. 14)
2) Use
the
condenser
height
adjustment
knob @
to move the condenser up and
down
until
an
image
of the field dia-
phragm
can
be
seen
clearly
in the eye-
pieces.
The
rotation of the knob
in the
direction
of the arrow lowers the con-
denser.
Field iris diaphragm image
Field of view
Fig. 15
Field Iris Diaphragm
@
The field iris diaphragm controls the diameter of the ray bundle impinging on the speci-
men
surface
and
therefore,
by
stopping
down
the field diaphragm
until
it is slightly
larger than the field of view, it can reduce stray light, which
in turn increases image defi-
nition and contrast.
Aperture
Iris Diaphragm
@
In order to achieve optimum
objective performance, the opening of the aperture iris
diaphragm
should be matched to the numerical aperture of the objective in use.
It is
often preferable,
however,
to stop down
the aperture diaphragm
slightly more than in-
dicated by the objective N.A.
This will result in better image contrast, increased depth of
focus and a flatter field.
@ After completing
focus adjustment, re-
<
B
70
— 80%
move one of the eyepieces from the obser-
;
p
7207508
vation
tube
and
look
into the empty
eyepiece tube. As you stop down the aper- DPening of the
ture iris diaphragm, the image of the iriş aperture diaphragm 人
diaphragm
can
be
seen
in the objective
pupil,
Adjust
the
opening
of the
dia-
phragm to match the N.A. of the objective
in use. If the specimen is low in contrast,
it is recommended
to stop down to 70%
~80% of the objective N.A. (Fig. 16)
Objective exit pupil
Fig. 16
12