Olympus BHTP 100 Instruction Manual - Page 14

Browse online or download pdf Instruction Manual for Microscope Olympus BHTP 100. Olympus BHTP 100 23 pages. Polarizing microscope

Olympus BHTP 100 Instruction Manual
Field diaphragm o l W
~ a n d u t l c ~
R&
diaphragm opened
and
centered.
6. Push analyzer El into
the light
path (Fig.
lo),
and
make sure that
both
polariter and analyzer
are
set at position
'0"
to attain the *crossed filter"
position. Then I m e n the clamping screw Q of the polariuer. (Fig. 12)
7.
Remove
the specimen
from the light path so
that a transparent area
comes
into the light
path.
Keeping
the polarizer at the
' 0 *
position, rotate
the potan'zer rotation ring E l until the
optimum
extinction is obtained,
then
clamp the ring.
(Fig.
12)
Make sure
t b t
no compensator plate is engaged.
E .
Cente~ng the S t a g e s e e diagrams a, b, c
1. Place a specimen on the stage and focus it. Looking through the
cross
hairs eyepiece and the 10X objective, fix your eye
on
a particular, easily
recognizable point of the specimen (point A). Bring this point (A)
to
the
center
o f
the cross hairs of the eyepiece.
2. Slowly rotate the stage, observing h e path taken by point A
as
the
stage is rotated 180' (point C ) and then a complete turn,
&W+WA.
(See
diagram a below). Visualize the imaginary center, E, of the
circular path traversed by the specimen as the stage is rotated a
complete turn. Rotate the stage
180"
again so that recognizable point
A
is now at the position
C
(See
diagram
a below).'
Using the stage eenbrlng knobs (Fig.
13 E l ) ,
bring the recognizable
point from position C to where the imagined Center E had previously
been
Iwated. (See
diagram b
below).
4.
Rotate
the
stage
a
complete
turn
while
observing
the
recognizable
point
(A).
If
the
movements
effected
in
steps
2
and
3
were
done
perfectly,
the
recognizable
point
would
now
travel
a
circte
with
the
crosshairs
as
the center
of
that
circle.
If
it
does
not,
repeat
steps
2
and
3
until
the stage
centration
is
perfected.
When
done,
any
point
of
the
specimen
in
view
will,
when
the
stage
is
completely
rotated,
travel
in
a
circle
w$bJbggoss
hairs
as
the
center
of
that
circle.
(See
diagram
c).
d i g w
%
diagram
b
diagram
c