Olympus BX-RFAA Instructions Manual - Page 31

Browse online or download pdf Instructions Manual for Microscope Olympus BX-RFAA. Olympus BX-RFAA 42 pages.

3. Push in the adjustment lever of the balancer slider to be used to engage the filter in the light path.

}The angle of each adjustment lever can be adjusted in the range shown below, only when the lever is pushed in.

4. While conducting fluorescence observation, adjust by tilting the adjustment lever of the exciter balancer which is currently

in the light path.

· With the U-EXBABG, setting the lever to 0° enhances the fluorescence of longer wavelengths (near red) and to 45°

enhances the fluorescence of shorter wavelengths (near green).

· With the U-EXBAUB, setting the lever to 0° enhances the fluorescence of shorter wavelengths (near blue) and to 45°

enhances the fluorescence of longer wavelengths (near green).

· With the U-EXBAUG, setting the lever to 0° enhances the fluorescence of longer wavelengths (near red) and to 45°

enhances the fluorescence of shorter wavelengths (near blue).

Observing a Triple Stained Specimen

}The operation is basically similar to the double stained specimens, but fluorescence mirror units for triple staining should

be used. The exciter balancers to be used are the U-EXBAUG (insertion slot 2) and U-EXBAUB (insertion slot 1).

· While conducting fluorescence observation, adjust the intensities of the three fluorescence lights by tilting the two adjust-

ment levers.

NOTES

1. When the adjustment lever of an exciter balancer is stood vertically, flare tends to occur easily due to the repeated

reflections on the filter surface. Be sure to disengage the exciter balancer from the light path when it is not used.

2. Be sure to stand the adjustment lever vertically when disengaging the filter from the light path or removing the exciter

balancer. (Otherwise, damage may result.)

3. To use the ND filters while the exciter balancer is already used, insert the ND filters in the 6-position filter insertion slot

which is on the further left.

Front (Observer)

U-EXBAUG

U-EXBABG or U-EXBAUB