Olympus BX3-25ND6 Instructions Manual - Page 13

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Dichroic Mirror and Filter Configurations of Fluorescence Mirror Units

Excitation

Mirror unit

method

U-FUW

U-FUN

U-FVN

U-FBVW

U-FBW

U-FBN

U-FGW

U-FYW

Combinations for color separation

U-FUNA

U-FBWA

U-FBNA

U-FGWA

U-FGNA

Exclusive combinations for fluorescent proteins

CFP

U-FCFP

GFP

U-FGFP

YFP

U-FYFP

RFP

U-FRFP

mCherry

U-FMCHE

Dichroic mirror

Excitation filter

BP340-390

DM410

BP360-370

DM455

BP400-410

DM455

BP400-440

BP460-495

DM505

BP470-495

DM570

BP530-550

DM600

BP545-580

DM410

BP360-370

BP460-495

DM505

BP470-495

BP530-550

DM570

BP540-550

DM455

BP425-445

DM490GFP

BP460-480

DM515

BP470-500

DM565HQ

BP535-555HQ

DM595

BP565-585

Barrier filter

· Autofluorescence observation

BA420IF

· DAPI: DNA staining

· Hoechest 33258, 33342: Chromosome

· Catecholamine observation

BA460IF

· Serotonin observation

· Tetracyline: Bones, teeth

· Quinacrine, quinacrine mustard:

Chromosome

BA460IF

· Thioflavine S: Lymphocyte

· Acriflavine: Nucleic acid

· ECFP

· FITC: Fluorescent antibody

· Acridine orange: DNA, RNA

BA510IF

· Auramine: Tubercle bacillus

· EGFP, S65T, RSGFP

· Rhodamine, TRITC:Florescent antibody

BA575IF

· Propidium iodide: DNA

· RFP

BA610IF

Texas Red: Fluorescent antibody

For observing only the U-excitation stain

BA420-460

when using U-excitation stain together

with FITC.

For observing only the B-excitation stain

BA510-550

when using B-excitation stain with TRITC

or Texas Red.

For observing only the G-excitation stain

BA575-625

when using G-excitation stain together

with Cy5.

BA460-510

BA495-540

BA515-560

BA570-625HQ

BA600-690

Applications

For ECFP.

For EGFP.

For EYFP.

For RFP.

For mCherry.