Olympus BXFM-ILH Instructions Manual - Page 19

Browse online or download pdf Instructions Manual for Microscope Olympus BXFM-ILH. Olympus BXFM-ILH 36 pages. Focusing unit

Print Page

5-3 Reflected Light Simplified Polarized Light Observation

}For the preparation of reflected light simplified polarized light observation, perform the operation in "1 Setting the Analyzer

and Polarizer" in section 5-2, "Reflected Light Nomarski DIC Observation".

Observation Method

Place the specimen on the stage plate and adjust the focus by using the coarse/fine adjustment knobs. Now simplified

polarized light observation can be started.

U-DICRH

1. Adjust the background contrast by turning the prism movement knob |

on the DIC slider as described below. (Fig. 18)

2. When the prism movement knob on the U-DICRH DIC slider is turned,

the interference color in the background varies from -100 to 100 nm. Set

the retardation which can provide best contrast.

· Selecting a gray background color enables 3D-looking observation with

high contrast thanks to the highest sensibility of the gray sensitive color.

· Selecting a magenta sensitive color allows even small phase variation to

be observed as a change in color.

To select the magenta sensitive color as the background color, use the

U-POTP3 polarizer and insert it so that the ( indication can be seen from

the front.

# Since the DIC observation has a high detection sensitivity, take care

against contamination on the specimen surface.

Switching to Brightfield Observation

1. Loosen the mounting knob @ on the front of the DIC revolving nose-

piece, gently pull out the DIC slider ² until it clicks, then clamp by tight-

ening the mounting knob.

2. Slide the analyzer (U-AN360-3) and polarizer to disengage them from the

light path.

BXFM

(Fig. 18)