Olympus CHD Instructions Manual - Page 12

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Diopter Adjustment (for the binocular tube)

1) Look at the image through the right eyepiece with your right eye,

and focus on the specimen with the focus adjustment knobs.

2) Next, looking at the image through the left eyepiece with your left

eye, rotate the diopter adjustment ring

to focus on the specimen

without using the focus adjustment knobs. (Fig. 13)

Fig. 13

Objective Selection

1) Swing in the objective to use. (Fig. 14)

2) Be certain to click the nosepiece in position.

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Fig.

Adjustment of the Aperture Iris Diaphragm

The opening of the aperture iris diaphragm built in the condenser

can be adjusted to match with the numerical aperture of the

objective in use, in order to achieve optimum objective perform-

ance such as oepth of focus, image contrast and resol ut ion.

1) Turning the diaphragm lever

counterclockwise reduces the dia-

phragm opening. (Fig. 15)

Fig. 15

Exit pupil

of objective

70% - 80%

30% - 20%

2) Remove the eyepiece, and looking at the exit pupil of the objective

through the empty eyepiece tube, adjust the opening of the dia-

phragm. Generally, it is preferable to stop down the aperture dia-

phragm to 70% to 80% of the objective N.A. (Fig. 16)

If the specimen is lightly stained, or almost colorless and transparent,

further reduce the diaphragm opening to increase contrast for better

observation. Be careful, however, if the diaphragm is stopped down

too much, the resolution will deteriorate.

Fig.

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