Olympus IX2-RFSPOT Instructions - Page 3

Browse online or download pdf Instructions for Microscope Olympus IX2-RFSPOT. Olympus IX2-RFSPOT 4 pages.

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Setting the pinhole diameter
The relationship between the actual pinhole diameter and the diameter of its projection on the specimen surface is as
expressed below.
Pinhole diameter on specimen = 2X (Pinhole diameter/Objective magnification)
(Example) When the pinhole diameter is 100 µm and objective magnification is 40X:
# If strict pinhole diameter setting is required, actually project it on a specimen and measure the diameter using
a scale.
Pinhole Design (Unit: mm)
Pinhole position
Microscope frame side
Relationship Between the Number of Washers and the Objective/Wavelength (This chart is for reference only.
Depending on the objective/wavelength, the pinhole image may not be brought into focus.)
UIS2 Series
Objective Type &
Magnification
UPlanFLN40X
UPlanSApo40X
PlanApoN60XO
UPlanSApo60XW
UPlanSApo60XO
UPlanSApo100XO
X: Unusable because of impossibility of focusing the pinhole.
* The priority can be changed between the brightness (condition in which light is gathered in the center of the field of
view) and the contrast (position where the pinhole appears sharpest although it looks a little dim) by adjustment of the
mercury lamp's collector lens.
2X (100/40) = 5 µm
Excitation Wavelength & Priority* (Brightness/Contrast)
340 nm
Brightness
Contrast
Number of Washers
X
X
X
X
X
X
X
0
0
0
0
1
1.1
Size indication surface of
common commercially
available pinhole
Lamp housing side
1.6
360 nm
Brightness
Contrast
Number of Washers
X
X
0
0
2
1
0
0
0
0
1
0
480 nm
Brightness
Contrast
Number of Washers
1
1
2
2
2
2
2
2
2
2
2
2