Olympus KHC Instructions Manual - Page 7
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c.
Focusing
1.
Turn the fine adjustment knob to bring the index line to the middle of the two lines
(movement range).
2.
Place the specimen on tne stage and secure it with the clips.
3.
Put the
lOX
objective in position.
4.
Looking from side of the microscope, move the stage upward carefully by the coarse
adjustment knob until the specimen is brought very close to the tip of objective.
(** Be most careful not to hit the objective.)
5.
Looking through the eyepiece, gradually lower the stage to obtain rough focus.
6.
Turn the nosepiece to set the desired objective in position. Accrately focus with the
fine adjustment knob.
7.
The specimen may be moved by the slide manipulation knob, built on the stage so
that the particular spot to be observed comes into the center of the view field.
****
For convenience in the later operations, tighten up the pre-set focus lock when the
specimen is roughly in focus at step 5, above. (See for V -A-2, Pre-set focus lock)
D. Inter-pupillary Distance and Dioptor Adjustments
1.
The interpupillary distance is adjusted by
a.
Holding both eyepiece tube seats,
b.
Looking into the eyepieces, and
c.
Pulling apart or pushing closer the seats until the view field is observed in one.
When the adjustment is made, read the figure on the scale; this will make the
adjustment easier from the next time on, as it can be done by merely setting the
tUbes at the given figure.
2. Diopter is adjusted by
a.
Looking through the right eyepiece with the right eye,
b.
Focusing on the specimen by the coarse and fine adjustment knobs,
c.
Then looking into the left eyepiece with the left eye, and
d.
Turning the diopter ring until the specimen is brought into focus correctly.
When looking into the left eyepiece with the left eye, do not touch the coarse or fine
adjustment knob.
E. Use of Oil Immersion Objective
First, bring the specimen into focus with a low magnification objective (lOX). Apply a
drop of cargille oil on the specimen as well as at the tip of the oil immersion objective.
Turn the nosepiece to bring the oil immersion objective above the specimen. This can be
done without the fear of crashing them into each other. Focus accurately with the fine
adjustment knob and make observations. Upon completion of observations, carefully wipe
off cargille oil on the oil immersion objective with a piece of gauze moistened with xylol.
****
Cargille oil left on the objective can ruin the lens greatly. An utmost care is
required in this point.
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