Olympus MIC-D Quick Start Manual - Page 10
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8
Illumination
Techni9_ue
_
There are four different ill umination techniques that you can use to
view your specimen or sample
Transmitted Light
Use this technique to view conventional microscope slides or especially
thin specimens. This techn
ique
is also known as
'Brightfield'.
1.
Rotate the Illumination Arm fully clockwise to the vertical position.
.
Rotate the Lamp Head fully counter
clockwise.
3.
Push in the Light Diffuser
Oblique
and
Darkfield
Ill
umination
Use either of these techniques on any transmitted light specimen or
sample to emphasize contrast. Use Oblique Illumination to highlight
structural detail by shadowing the 'hills' and
'valleys'.
Use Darkfield
to create a black background and to emphasize specimen
color.
1.
Rotate the Lamp Head
counter-clockwise.
2.
Pull out the Light
Diffuser.
Adjustments to the light diff-
user position may be
required.
3.
Slowly rotate the Illumination Arm
counter-clockwise:
- For Oblique Illumination rotate the arm until the back-
ground just begins to
darken.
Three dimensional details
should display a shadowing effect.
- For Darkfield Illumination rotate the
Illumination
Arm
further
counter-clockwise.
Stop when the background
turns black. Best results are achieved at high
magnification.