Olympus U-CSE Instructions Manual - Page 12

Browse online or download pdf Instructions Manual for Microscope Olympus U-CSE. Olympus U-CSE 24 pages.

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U-CBE, U-CTB

Fig. 3

Measuring Retardation

} If an interference filter (IF 546 or IF 550) is available, use it

in this measurement.

1. Place an interference filter in the filter holder of the microscope

frame.

# If an interference filter is used, multiple black dots or

fringes will be visible. Use the black dot or fringe that

is visible when the interference filter is not used.

To identify this dot or fringe, check the position of

the black dot or fringe without using the filter before

engaging it, then engage the interference filter and

confirm the same black dot.

2. Rotate the compensator knob to the position where the

measured portion on the center of the field of view is darkest

(Fig. 3) Find the angle at this position by totaling the readings

of scales 1 and 2. → θ

3. Rotate the compensator knob in the opposite direction and

read the angle of the position with which the measured

portion on the center of the field of view is darkest in the

same manner as in step 2 above. → θ

4. Repeat steps 2 and 3 several times, obtain the mean value

of θ

, θ

, and that of θ

value θ using the following formula:

, θ

, and finally find the overall mean

│

– θ

│

θ =