Olympus U-LH100HG Instructions Manual - Page 25

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17. For red fluorescent protein (RFP)

18. For mCherry

U-FRFP

U-FMCHE

Wavelength (nm)

Typical Example of Emission Spectrum of Ultrahigh-Vacuum

Mercury Burner Spectrum

Wavelength (nm)

For fluorochrome emission, a light beam having a specific wavelength is selected from a

wide spectrum of wavelengths. The five major peaks of luminance are at wavelengths of

365/366, 404.7, 435, 546.1 and 577.0/579.1 nm. In addition, light beams having wavelengths

of 334.2 and 490 nm (at rather low luminance) are also applicable to fluorochrome

emission.