Olympus U-LH100HGAPO Instructions Manual - Page 12

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Olympus U-LH100HGAPO Instructions Manual
Dichroic Mirrors and Filter Configurations of Fluorescence Mirror Units
Excitation
Mirror Unit
Method
U-MWU2
U
U-MNU2
V
U-MNV2
U-MWBV2
BV
U-MNBV2
U-MWB2
B
U-MNB2
U-MSWB2
U-MWIB2
IB
U-MNIB2
U-MWG2
G
U-MNG2
U-MSWG2
IG
U-MWIG2
IY
U-MWIY2
Color Separation Filter Combinations
U
U-MNUA2
U-MWIBA2
IB
U-MNIBA2
Meaning of Mirror Unit Name
U - M N I B A 2
Mirror unit
Universal
Dichroic Mirror
Excitation Filter
BP330-385
DM400
BP360-370
DM455
BP400-410
BP400-440
DM455
BP420-440
BP460-490
DM500
BP470-490
BP420-480
BP460-490
DM505
BP470-490
BP510-550
DM570
BP530-550
BP480-550
DM565
BP520-550
DM600
BP545-580
DM400
BP360-370
BP460-490
DM505
BP470-550
Model number
For color separation
Excitation (U, UV, BV, B, IB, G, IG or IY)
Bandwidth (SW: Superwide band. W: Wide band. N: Narrow band.)
Barrier Filter
· Autofluorescence observation
· DAPI staining: DNA
BA420
· Hoechest 33258, 33342 staining: Chro-
mosome
· Catechrolamine observation
BA455
· Serotonin observation
· Tetracyine staining: Bones, teeth
· Quinacine & quinacine mustard stain-
ing: DNA
BA475
· Thioflavine S staining: Lymphocite
· Acriflavine staining: Nucleic acid
· CFP
· FITC staining: Fluorescent antibody
· Acridine orange staining: DNA, RNA
BA520IF
· Auramine staining: Tubercle bacteria
· EGFP, S65T, RSGFP
BA510IF
· Rhodamine & TRITC staining: Fluores-
cent antibody
BA590
· Propidium iodide staining: DNA
· RFP
BA580IF
BA610IF
Texas Red staining: Fluorescent antibody
For observing only the U-excitation stain,
BA420-460
when using U-excitation stain together
with FITC.
For observing only the B-excitation stain,
BA510-550
when using B-excitation stain together
with FRITC.
Fluorochrome
9