Olympus U-RFL-T Instructions Manual - Page 10
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REFLECTED FLUORESCENCE OBSERVATION PROCEDURE
} If you need simultaneous observation of reflected fluorescence observation with the phase contrast observation or
transmitted light Nomarski Differential Interference Contrast (DIC) observation, please read Chapter 4, "SIMULTANEOUS
FLUORESCENCE OBSERVATION". (Page 18)
Preparation
· Attach the fluorescence mirror unit and objective matching the observation method.
· Center the mercury burner.
Set the main switch to " I " (ON) and wait for the
lamp brightness to stabilize (5 to 10 minutes
after ignition).
Place the specimen on the stage.
Engage the fluorescence mirror unit matching
the specimen in the light path.
Engage the objective in the light path and focus
on the specimen
BX3-URA
Adjust so that the
entire field is uniform
and brightest.
Observation
} Engage the shutter if you interrupt observation for a short time.
7
Engage an ND filter in the
light path as required.
BX3-RFAS
Adjust the brightness
as required.
Adjust the field iris diaphragm.
Adjust the aperture iris
diaphragm.
(Controls Used)
@Main switch
2Slide holder
3X-/Y-axis knobs
4Mirror unit turret
5Revolving nosepiece
6Coarse/fine adjustment knobs
7ND filter
8Collector lens focusing knob
9Field iris diaphragm knob
aAperture iris diaphragm knob
bShutter knob
(Page)
(Pages 9 to 10)
(Pages 13 to 15)
(P.11)
(P.16)
(P.13, 1 4)
(P.11)
(P.12)
(P.11)