Olympus R1 Instructions Manual - Page 44

Browse online or download pdf Instructions Manual for Laboratory Equipment Olympus R1. Olympus R1 47 pages. Cell counter
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6.4 Troubleshooting

Problem

Too few or too many cells

Inaccurate cell

count

Improper sample loading

Damaged or contaminated

Incompatible USB memory

Data transfer

and saving

Too many files in the USB

Incompatible USB memory

Errors while

updating or

More than one software

calibrating the

instrument

LED on printer

Paper receptacle empty

flashing

Possible Cause

Clumped cells

Improper insertion of

counting slide

Malfunction of optical

components

counting slide

memory

Freezing during

background calibration

version on the USB

memory

Incorrectly saved or

damaged software

Gently but thoroughly pipette your cell suspension to

break up aggregates prior to counting. Alternately,

increase trypsinization time.

Cell concentrations of 5 x 10

for counting. Dilute or concentrate cell suspensions

accordingly.

Ensure that the Cell Counting Slide has been inserted

properly into the instrument.

Do not over- or under-fill the Cell Counting Slide

chambers. Carefully load the chambers with 10-12 µL of

cell suspension.

Optical components may be dirty or damaged. Please

contact Olympus.

Use a new Cell Counting Slide. Wear gloves and handle

by the edges to avoid smudging and contamination.

Some USB memory devices are undetectable or

incompatible. We recommend that you use the USB

memory provided with this product.

Delete or transfer files.

If calibration takes more than 10 minutes, reset the

system by turning the power off and then on again.

Contact Olympus if calibration fails repeatedly.

Some USB memory devices are undetectable or

incompatible. We recommend that you use the USB

memory provided with this product.

Delete previous versions of software from the USB

memory before downloading new software.

Use the USB memory supplied or make sure that your

USB is compatible with the instrument. Download the file

again into the root directory of the USB memory. Insert the

USB properly. Press [Software Updates] in the Settings

screen. If the problem persists, contact Olympus.

Check to see there is enough paper in the printer paper

receptacle. If there is not, replace the printer paper (see

Section 6.3: Installing Printer Paper). If the flashing

persists, contact Olympus.

Solution

-1 x 10

cells/mL are optimal