Olympus U-PCD2 Instructions Manual - Page 6

Browse online or download pdf Instructions Manual for Laboratory Equipment Olympus U-PCD2. Olympus U-PCD2 8 pages. Phase contrast condenser

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Phase Contrast Observation

1. Swing the objective of your choice into the light path.

2. Rotate the phase contrast turret until the turret index dot indicates the Ph number seen through the

central window which corresponds to the Ph number indicated on the side of objective lens.

3. Focus roughly on the specimen.

4. Remove one of the eyepieces, and insert the centering telescope U-CT30 into the eyepiece sleeve.

5. Rotate the top lens assembly of the U-CT30 to bring the bright ring (light annulus in the condenser) and

the dark ring (phase annulus in the objective) in focus.

6. Rotate the two light annulus centering knobs @ of the condenser until the light annulus and phase

annuls are concentric and superimposed. (Fig. 3)

Fig. 3

Before centration

After centration

a = b = c = d