Olympus U-RFLT50 Instructions Manual - Page 13

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Olympus U-RFLT50 Instructions Manual
2
Selecting the Reflected Light Fluorescent Mirror
}The reflected light fluorescent mirrors for B-excitation and G-excitation have been installed in the
fluorescent mirror slider at the factory.
If required, the optional CKX-NU mirror for U-excitation can be mounted in the open position or by
removing the B- or G-excitation mirror. (Work to be done by the dealer)
Fluorescent mirror specifications
Excitation Fluorescent Mirror Dichroic Mirror Excitation Filter
B
G
U
CKX-NU
Spectral characteristics
DM500
BP460-490C
DM570
BP480-550C
DM400
BP360-370
B-excitation
Wavelength nm
U-excitation
Wavelength nm
REFLECTED FLUORESCENCE SYSTEM
Barrier Filter
· FITC: Fluorescent antibody method
· Acidine orange: DNA, RNA
BA520 IF
· Auramine: Tubercle bacillus
· EGFP, S65T, RSGFP
· Rhodamine, TRITC: Fluorescent anti-
body method
BA590
· Propidium iodide: DNA
· RFP
· Auto-fluorescence observation
· DAPI: DNA
BA420
· Hoechest 33258, 33342: Chromosome
G-excitation
Wavelength nm
FOR CKX41
Applications
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