Nikon A1+ Standard Operating Procedure - Page 3

Browse online or download pdf Standard Operating Procedure for Microscope Nikon A1+. Nikon A1+ 15 pages. Lbic nikon confocal
Also for Nikon A1+: User Manual (28 pages)

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Finding your sample

1. Put your sample in the appropriate holder, if you have slides make

sure the coverslip is facing down.

2. Start with either 10x or 20x objective

3. Navigate to a suitable place in your sample with the joystick.

4. Press "Eye port" to change the light path to the eye piece, if

needed press the Sola button (on Sola pad) to activate the LED

lights.

5. Use the coarse focusing mode and find focus (for slides around Z=

2100-2200, for 30mm petri dishes around Z=2500-2700)

6. Press "Eye port" again to switch to confocal mode (the first time

the system is started do this twice, this is a bug in the software).

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