Nikon Eclipse E600 Instructions Manual - Page 20

Browse online or download pdf Instructions Manual for Microscope Nikon Eclipse E600. Nikon Eclipse E600 37 pages. Teaching head/multi teaching head
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3. Operation of Each Part

3. Objective and condenser combinations

ondenser

та

Swing-out

Achromat

Abbe

Objective

aplana

condenser

magnification

condenser

O (Note 1) | O (Note 1)

10x to 100x

©: Appropriate

— X: Inappropriate

Note 1: The entire view field may not be satisfactory when a UW eyepiece is used.

Note 2: Indoor lighting and similar light sources may be reflected from the surface of the condenser

lens and be visible within the view field. When this happens, either dim the indoor lighting

or prevent strong outside light from entering the stage.

Note 3: Swing out the top lens before usage.

¢ Depending on the type of objective, the whole numerical aperture of the objective may not be

covered.

For example, when an objective with an N.A. of 1.4 is used, the aperture size of the

swing-out condenser or the abbe condenser will only be about 65% of the objective's N.A. even

when it is fully opened.

* Refer to the condenser instruction manual for details of universal and phase contrast condensers.

Field diaphragm

The field diaphragm restricts illumination to the area on the specimen being viewed.

Turning the

field diaphragm ring changes the size of the field diaphragm.

For normal observation, the size of the

diaphragm should be slightly larger than the boundary of the view field.

If a broader area than

necessary is illuminated, stray light will enter the view field, creating flaring and reducing the con-

trast of the optical image.

The correct setting of the field diaphragm is especially important in

photomicrography; generally, good results are obtained by stopping down the field diaphragm to

just slightly larger than the area that will be reproduced on the film, i.e., the size of the photo frame.