Nikon Labophot Instruction Manual - Page 10
Browse online or download pdf Instruction Manual for Microscope Nikon Labophot. Nikon Labophot 28 pages. Y-r stand biological microscope
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Vertical photo
tube 100%
Eyepiece
viewfield stop
Eyepiece
viewfield stop
Image of field
diaphragm
"",r
Image of field
'\
diaphragm
---
denser vertically
so that
a sharp image of
the field diaphragm
is formed
on the speci-
men surface.
(2)
Bring
the
field
diaphragm
image
to the
center of the field of view by means of the
condenser
centering
screws. (Fig. 12-W)
(3)
Change
over
to
the
40X
objective,
and
adjust
the
field
diaphragm
so that
the
image of the diaphragm
is about
the same
as that of the field of view, as shown in Fig.
12-[Z].
If not centered,
use the condenser
centering
screws again.
Fig. 9
Fig. 10
Optical path
change-over
knob
Observation
tube
100"10
(2)
When
using the
trinocular
eyepiece
tube
"T"
or
the
ultra
wide
eyepiece
tube
"UW"
As shown
in Fig. 10, with
the change-over
knob
pushed
in, 100% of light
enters the
observation
tube.
As shown
in Fig. 11, with
the change-over
knob
drawn
out,
the
proportion
of light
entering
the
binocular
observation
tube
and vertical
photo tube will be 14 : 86.
Observation tube:
vertical photo
tube = 14:86
Optical path
change-over
knob
Fig. 11
4) Centering the condenser
lens
(1)
Close the field diaphragm
in the microscope
base to its smallest
size by means of the
field
diaphragm
control
ring.
Rotate
the
condenser
focus
knob
to move
the con-
Fig. 12
5) Use of condenser
aperture diaphragm
The condenser
aperture
diaphragm
is provided
for adjusting
the numerical
aperture
(N.A.)
of
the
illuminating
system
of
microscope.
It is
important
because it determines
the resolution,
contrast
and depth of focus.
In general,
when
it is stopped
down
to 70 ~
80% of the numerical
aperture
of the objective,
a good
image of appropriate
contrast
will
be
obtained.
(Fig. 13)
Objective pupil
Aperture diaphragm
Size of the condenser aperture diaphragm
Fig. 13
10