Nikon Live Cell 2 Manual - Page 11

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Large images

1. Tiled images can be captured by selecting large

image in ND Acquisition. (Fig. 26)

2. Set the number of fields for the large image,

select stitch and set overlap to 15%.

3. Click 'run now' to acquire. If selected alongside

timelapse, make sure that large image

acquisition loop fits into frame interval via the

timing button.

4. A more advanced method of tiling can be

accessed through Acquire>Scan Large image on

the upper menu bar.

5. Select the objective for capturing the image, set

the number of fields in XY and whether the tiling

should scan around the current position of the

stage or with the stage position being the top

left. Set the overlap to 15% and select blending

(making sure image registration is checked). (Fig

27)

6. Large image files can either be saved as the

whole large image, the single frames (as TIFFs)

or both. Set your folder for saving and file name

for the save option required.

7. Z-stacks may also be applied to the image as

well as automatic refocusing throughout the

acquisition. Once all options have been

adjusted, click scan.

Nikon Imaging Centre @ King's College London

Nikon

King's College London

Imaging Centre

Fig. 26